Electrophoretic studies of induction in neurospora

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Joyce Lee Barwick (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
William Bates

Abstract: Alterations In soluble protein levels of Neurospora following the addition of galactose were studied by disc electrophoresis on polyacrylamide gels. The isolation of a strain which shows improved growth characteristics on glycerol has allowed the development of a system in which galactose can be used as an inducer under conditions in which glycerol is preferentially utilized as the carbon source. A comparison of lactose and galactose showed lactose to be ineffective as an inducer. Isopropyl-B-D-thiogalactopyranoside did not induce B-galactosldase at the concentrations tested. An attempt to establish gratuitous conditions by isolating a mutant strain which could not metabolize galactose but could be induced to synthesize B-galactosidase was abandoned when the nature of the mutations could not be identified by the techniques available.

Additional Information

Publication
Thesis
Language: English
Date: 1969
Subjects
Neurospora $x Experiments
Proteins $x Experiments

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