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Identification and stability of anthocyanins in Plantago lanceolata

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Stacy Dee (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Nadja B Cech

Abstract: "Several laboratories have analyzed the content and stability of anthocyanins from foods and flowering plants ranging from potatoes to petunias. Previous research in the Cech and Lacey laboratories tentatively identified anthocyanins in Plantago lanceolata using reversed phase high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS). The goal of this research was to gain more information about the identification of these anthocyanins using hydrolysis and nuclear magnetic resonance (NMR) and to test the stability of these anthocyanins when stored for various periods of time at three different temperatures (room temperature, -20, -80 °C). Repeatability studies were also conducted to validate the analytical method. Hydrolysis experiments and analysis by HPLC-ESI-MS confirmed the presence of cyanidin and peonidin glycosides in the original P. lanceolata extract. The most abundant anthocyanin was isolated from P. lanceolata, but attempts to collect an NMR spectrum of this sample were not successful due to the small yield and apparent low purity of the sample isolated. The method variability from injection to injection on the same day was determined to be 10 - 20 %, variability from day to day on the same extract was 30 % and the variability from extraction to extraction on different days was 30 - 60 % for cyanidin glycosides a and c, delphinidin glycoside b, peonidin glycoside a, and petunidin glycoside b. Stability studies revealed that there was no degradation that exceeded the variability of the method from day to day in both the -20 and -80 oC temperatures for any of the anthocyanins. There was however, significant degradation in the room temperature condition for the cyanidin glycosides a and c."--Abstract from author supplied metadata.

Additional Information

Publication
Thesis
Language: English
Date: 2007
Keywords
laboratories, anthocyanins, flowering plants, Plantago lanceolata, electrospray ionization mass spectronomy, mass spectronomy, chemistry, biochemistry
Subjects
Plantago
Anthocyanins
Hydrolysis