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Metabolomic Evaluation of Di-n-butyl Phthalate-Induced Teratogenesis in Mice

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Wei Jia, Professor and Co-Director of the UNCG Center for Research Excellence in Bioactive Food Components (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: Di-n-butyl phthalate (DBP) has been linked to the neural, reproductive and developmental toxicity. We present here a metabolomic study that characterized the metabolic variations associated with the DBP-induced teratogenesis in maternal and fetal mice. DBP at 50 and 300 mg/kg were administrated to pregnant C57 mice, via gastric intubation on gestation day 7–9, respectively. Maternal mice were euthanized on gestation day 16 and examined for fetal development and malformations. Metabolomic study of maternal serum, placenta and fetal brain tissues was performed using gas chromatography time-of-flight mass spectrometry combined with multivariate data analysis (MVDA). The results showed that a 50 mg/kg dose of DBP had no significant effect on fetal development and a 300 mg/kg dose caused embryo resorption and fetal malformations (primarily eye abnormalities and encephalocele). MVDA indicated that DBP at two doses gave rise to disruption of maternal and fetal metabolic profiles characterized by significantly altered tricarboxylic acid cycle, amino acid, purine and lipid metabolism.

Additional Information

Publication
Metabolomics, 7(4), 559-571
Language: English
Date: 2011
Keywords
metabolomics, di-n-butyl phthalate, developmental toxicity, neural tube defects, gas chromatography time-of-flight mass spectrometry