Anti-SPARC Oligopeptide Inhibits Laser-Induced CNV in Mice

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Ethan W Taylor, Senior Research Professor (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: It is known that SPARC gates VEGF-A signal transduction towards KDR, the primary angiogenic VEGF receptor. We sought to determine whether inhibition of SPARC activity using anti-SPARC peptide could inhibit laser-induced CNV by promoting binding of VEGF-A to FLT-1. We created anti-SPARC L-peptide and retro-inverso anti-SPARC D-peptide. Anti-SPARC peptides or PBS were injected intravitreally one day before or after laser induction. Intravitreal injection of anti-SPARC L-peptide one day before laser induction promotes Flt-1 phosphorylation and inhibited laser-induced CNV and anti-SPARC D-peptide had no effect. Injection one day after laser injury did not affect size of laser-induced CNV. Inhibition of SPARC activity could be complementary to existing anti–CNV therapy.

Additional Information

Publication
Vision Research, 50(7), 674-679
Language: English
Date: 2010
Keywords
age-related macular degeneration (AMD), choroidal neovascularization (CNV), SPARC, FLT-1, VEGF