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Developmental Effects of a Chimeric ultraspiracle Gene Derived From Drosophila and Chironomus

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Vincent C. Henrich, Professor (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: The ultraspiracle (usp) gene encodes a nuclear receptor that forms a heterodimer with the ecdysone receptor (EcR) to mediate transcriptional responses to the insect steroid hormone, 20-hydroxyecdysone (20HE). The responses ultimately elicit changes associated with molting and metamorphosis. Although Ultraspiracle (USP) is required at several developmental times, it is uncle: whether USP plays stage-specific roles in Drosophila. A chimeric transgene (d/cusp), produced by replacing the ligand-binding domain (L11311)) of Drosophila USP with the equivalent domain from another 11)iptera, Chironomus tentans, was tested for its ability to rescue Drosophila usp mutants from early larval lethality. A single copy of the d/cusp was sufficient to rescue transformants from several lines through larval development but they died suddenly during the late third instar. Additional doses of d/cusp were required to allow survival through the adult stage, but they did not restore a normal prepupal contraction. Thus, the arrest at the onset of metamorphosis apparently is caused by the impaired ability of the chimeric USP to mediate a stage-specific function associated with the L11311).

Additional Information

Publication
Genesis, 28:125-133.
Language: English
Date: 2000
Keywords
Ecdysteroid, Nuclear receptor, Heterodimerization, Metamorphosis, Chimera, Retinoid X receptor (RXR)