The effect of 17 B-estradiol and vitamin A on the proliferation of the cell line I-407

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Kathryn Louise Johnson (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Laura Anderton

Abstract: Cultured intestinal epithelial cells (I-407) and standard tissue culture techniques were used to study the following: a) the necessity of changing culture medium the day following subculture; b) the normal growth curve of an early and older passage of cells; c) the alterations in the growth curve of the cells after a 1 hour pulse or exposure with 17 B-estradiol, vitamin A, estradiol-vitamin A combination, alcohol control, and untreated control; and d) differences in cellular response to therapeutic and ethanol-diluted sources of 17 B-estradiol. Determinations of the number of viable and non-viable cells/ml in cell suspensions were made involving cellular fixation with a 2.5% solution of glutaraldehyde. Cell proliferation was determined by hemocytometer counts. Concentrations of 17 B-estradiol and vitamin A were chosen for this in vitro study based on normal physiological levels. A review of the literature pertaining to these test substances and procedures for determining cell growth and proliferation is included. The critical point in cell proliferation when the culture medium was not changed was found to be at 48 hours. Growth curves for cells cultivated under normal conditions were plotted for periods of 72 hours. Control and alcohol control cell treatments exhibited a significant difference only at the 48 hour point after the 1 hour exposure.

Additional Information

Language: English
Date: 1974
Cells $x Effect of drugs on
Cells $x Growth
Cells $x Regulation

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