Effects of estrogen on the morphology of human chromosomes and on the mitotic rate of chromosomes in vitro
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Elizabeth Ann Barrow (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- Laura Anderton
Abstract: An investigation was made to determine the effect of the natural estrogen estrone, in varying concentrations and over varying periods of time, on the chromosomes of human female peripheral blood lymphocytes in vitro. All experiments were carried out according to a modification of Tips' Micro Procedure for Peripheral Leucocyte Culture. One set of leucocyte cultures was exposed to 6.0 u g of estrone for 24, 48 and 72 hours, and another set of cultures was exposed to 0.6 u g of the hormone for the same respective time periods. Chromosome spreads obtained from each culture were analyzed in respect to control groups for changes in morphology and mitotic rate. 100 control and 50 estrogen-treated spreads were analyzed for each experimental group in consideration of morphology, and 500 control and 500 estrogen-treated spreads were analyzed for each group in consideration of mitotic rate.
Effects of estrogen on the morphology of human chromosomes and on the mitotic rate of chromosomes in vitro
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Created on 1/1/1967
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Additional Information
- Publication
- Honors Project
- Language: English
- Date: 1967