De Novo Transcriptome Assembly and Comparative Analysis Elucidate Complicated Mechanism Regulating Astragalus chrysochlorus Response to Selenium Stimuli

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)
Şule Arı (Creator)
Özgür Çak?r (Creator)
Neslihan Turgut-Kara (Creator)
Baohong Zhang (Creator)
Institution
East Carolina University (ECU )
Web Site: http://www.ecu.edu/lib/

Abstract: Extracted text; Astragalus species are medicinal plants that are used in the world for years. Some Astragalus species are known for selenium accumulation and tolerance and one of them is Astragalus chrysochlorus, a secondary selenium accumulator. In this study, we employed Illumina deep sequencing technology for the first time to de novo assemble A. chrysochlorus transcriptome and identify the differentially expressed genes after selenate treatment. Totally, 59,656 unigenes were annotated with different databases and 53,960 unigenes were detected in NR database. Transcriptome in A. chrysochlorus is closer to Glycine max than other plant species with 43,1 percentage of similarity. Annotated unigenes were also used for gene ontology enrichment and pathway enrichment analysis. The most significant genes and pathways were ABC transporters, plant pathogen interaction, biosynthesis of secondary metabolites and carbohydrate metabolism. Our results will help to enlighten the selenium accumulation and tolerance mechanisms, respectively in plants.

Additional Information

Publication
Other
PLoS ONE; 10:10 p. 1-16
Language: English
Date: 2015
Keywords
Selenium, Auxins, Gene ontologies, Astragalus

Email this document to

This item references:

TitleLocation & LinkType of Relationship
De Novo Transcriptome Assembly and Comparative Analysis Elucidate Complicated Mechanism Regulating Astragalus chrysochlorus Response to Selenium Stimulihttp://hdl.handle.net/10342/5449The described resource references, cites, or otherwise points to the related resource.