An examination of Crassostrea virginica nuclear DNA variation along the North Carolina coast
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Russ Peterson (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
- Advisor
- Ami Wilbur
Abstract: Inhabiting coastal waters from eastern Canada to the Gulf of Mexico, the eastern oyster is
subjected to a wide range of temperature and salinity regimes, thus providing an interesting
opportunity to study population structure. Prior studies have examined phenotypic as well as
DNA differences along this range. A previous mtDNA population survey of Crassostrea
virginica within Pamlico Sound utilizing a single 16s polymorphism diagnostic for North
Atlantic / South Atlantic haplotypes revealed an ~110 km cline along the North Carolina coast.
Using 4 microsatellite loci, 3 SNPs and 1 scnDNA RFLP, I have surveyed eight oyster
populations within and outside the Pamlico Sound in an effort to corroborate the population
structure found in the mitochondrial genome. Three microsatellite loci were out of HWE across
populations vs. only 1 population for one SNP loci, and it seems likely that those microsatellite
loci were plagued with null alleles. Microsatellite exact tests show some significant differences
within the Pamlico Sound, mostly in comparisons involving the Stumpy Point population. A
combined SNP/RFLP analysis did reveal significant differences among populations, though most
of this can be accounted for by inclusion of a population from Maryland. The clinal structure
seen in the mitochondrial genome is not reflected in the nuclear genome within the Pamlico
Sound.
An examination of Crassostrea virginica nuclear DNA variation along the North Carolina coast
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Created on 1/1/2009
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Additional Information
- Publication
- Thesis
- A Thesis Submitted to the University of North Carolina at Wilmington in Partial Fulfillment of the Requirement for the Degree of Masters of Science
- Language: English
- Date: 2009
- Keywords
- American oyster--North Carolina--Genetics, American oyster--Variation--North Carolina, Oyster culture--Variation--North Carolina
- Subjects
- American oyster -- Variation -- North Carolina
- Oyster culture -- Variation -- North Carolina
- American oyster -- North Carolina -- Genetics