Investigation of protein-induced formation of lipid domains and their dynamics using fluorescence energy transfer
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Jenny R. Wright (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
- Advisor
- Paulo Almeida
Abstract: An important feature in the current understanding of membrane structure is the existence
of lipid domains. Lipid domain formation was observed in an experimental lipid bilayer system
containing an equilibrium mixture of glycerophospholipids and raft lipids. Protein-lipid
interactions in the equilibrium mixtures were observed when a peripheral membrane peptide was
added. These interactions were measured using fluorescence energy transfer. Domain formation
as a function of time was also investigated after the protein was added to the equilibrium mixture
using stopped-flow fluorescence. Domain formation in vesicles containing 40% anionic lipid
occurred in less than 1 s.
Domain dissipation was observed with stopped flow fluorescence spectroscopy in
glycerophospholipid vesicles as well as in a mixture of glycerophospholipids and raft lipids by
removing the peptide. Domain dissipation in vesicles containing glycerophospholipids with 20%
anionic lipid occurred in approximately 5 s. Domain dissipation in vesicles containing rafts and
40% anionic lipid occurred in approximately 900 s and domain dissipation in vesicles containing
rafts and 10% anionic lipid occurred in approximately 160 s. These timescales were compared to
the off-rate of the peptide from the vesicles.
Investigation of protein-induced formation of lipid domains and their dynamics using fluorescence energy transfer
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Created on 1/1/2009
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Additional Information
- Publication
- Thesis
- A Thesis Submitted to the University of North Carolina Wilmington in Partial Fulfillment Of the Requirements for the Degree of Master of Science
- Language: English
- Date: 2009
- Keywords
- Analytical biochemistry, Bilayer lipid membranes--Analysis, Fluorescence spectroscopy, Proteins--Analysis
- Subjects
- Fluorescence spectroscopy
- Analytical biochemistry
- Bilayer lipid membranes -- Analysis
- Proteins -- Analysis