Enhanced Avidity Maturation of Antibody to Human Immunodeficiency Virus Envelope: DNA Vaccination with gp120-C3d Fusion Proteins

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Thomas D. Green (Creator)

David C. Montefiori (Creator)

Harriet L. Robinson (Creator)

Ted M. Ross (Creator)

Yan Xu (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: DNA vaccination can elicit both humoral and cellular immune responses and can confer protection against several pathogens. However DNA vaccines expressing the envelope (Env) protein of human immunodeficiency virus (HIV) have been relatively ineffective at generating high titer long-lasting neutralizing antibodies in a variety of animal models. In this study we report that fusion of Env and the complement component C3d in a DNA vaccine enhances the titers of antibody to Env. Plasmids were generated that expressed a secreted form of Env (sgp120) from three isolates of HIV and these same forms fused to three tandem copies of the murine homologue of C3d (sgp120-3C3d). Analyses of titers and avidity maturation of the raised antibody indicated that immunizations with each of the sgp120-3C3d-expressing DNAs accelerated both the onset and the avidity maturation of antibody to Env. Originally published AIDS Research and Human Retroviruses Vol. 17 No. 9 June 2001

Additional Information

Publication

Other

AIDS Research and Human Retroviruses. 17:9(June 2001) p. 829-835.

Language: English

Date: 2011

Keywords

DNA vaccination, titers, avidity maturation

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