Delta-Catenin Induced Dendritic Morphogenesis: An Essential Role of p190RhoGEF Interaction through Akt1 Mediated Phosphorylation

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Sunghoe Chang (Creator)

Jeong Ran Han (Creator)

Sarah E. James (Creator)

Hyunkyoung Ki (Creator)

Hangun Kim (Creator)

Kim Kwonseop (Creator)

Kwang Youl Lee (Creator)

Qun Lu (Creator)

Minsoo Oh (Creator)

Jaejun Park (Creator)

Woo-Joo Song (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: delta-Catenin was first identified through its interaction with Presenilin-1 and has been implicated in the regulation of dendrogenesis and cognitive function. However the molecular mechanisms by which delta-catenin promotes dendritic morphogenesis were unclear. In this study we demonstrated delta-catenin interaction with p190RhoGEF and the importance of Akt1-mediated phosphorylation at Thr-454 residue of delta -catenin in this interaction. We have also found that delta-catenin overexpression decreased the binding between p190RhoGEF and RhoA and significantly lowered the levels of GTP-RhoA but not those of GTP-Rac1 and -Cdc42. delta-Catenin T454A a defective form in p190RhoGEF binding did not decrease the binding between p190RhoGEF and RhoA. delta-Catenin T454A also did not lower GTP-RhoA levels and failed to induce dendrite-like process formation in NIH 3T3 fibroblasts. Furthermore delta-catenin T454A significantly reduced the length and number of mature mushroom shaped spines in primary hippocampal neurons. These results highlight signaling events in the regulation of delta-catenin-induced dendrogenesis and spine morphogenesis. Originally published Journal of Biological Chemistry Vol. 283 No. 2 Jan 2008

Additional Information

Publication

Other

Journal of Biological Chemistry. 283:2(January 2008) p. 977-987.

Language: English

Date: 2011

Keywords

delta-catenin, Phosphorylation, dendritic morphogenesis

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