MicroRNA profiling in the left atrium in patients with non-valvular paroxysmal atrial fibrillation

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Jiangang,Song,Shiqiu,Xie,Changqing,Han,Jie,Li,Yan, Wang (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: identifying miRNAs that were significantly associated with non-valvular paroxysmal AF.Methods: The RNA samples were isolated from healthy controls (n = 5) and patients with atrial fibrillation (n = 8). Toconfirm the findings obtained by analyzing the miRNA profile, we measured the expression of selected miRNAs inthe entire cohort by quantitative PCR.Results: Ten specific miRNAs were found to be differentially expressed between atrial fibrillation and healthy controlswith more than a 2-fold change (P < 0.05). Consistent with the data obtained for the profile, expression levels ofmiRNA-155, miRNA-146b-5p and miRNA-19b were significantly increased in patients with atrial fibrillation. Interestingly,levels of miRNA-146b-5p and miRNA-155, which are known to be associated with inflammation, were independentlyand positively associated with left atrium dimension, atrial fibrillation duration and high sensitivity C-reactive proteinlevels. By using four Databases (TargetScan, miRanda, Starbase Clip-seq and miRDB) to perform target gene prediction,there were four genes were related to the inflammatory response and fibrosis, and three others encoding cardiac ionchannel proteins. As a result of TaqMan qPCR and Western analysis, the relative mRNA and protein expression level ofthree target genes (DIER-1, TIMP-4 and CACNA1C) were significantly lower in the atrial fibrillation group than that inthe healthy control group.Conclusions: Expression of inflammation-associated miRNAs is significantly up-regulated in the left atrial appendage ofpatients with

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Language: English

Date: 2015

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