Development Of 17 Microsatellite Markers In The Federally Endangered Species Liatris helleri (Asteraceae)

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Logan Clark (Creator)
Matt Estep, Assistant Professor (Creator)
Morgan Gaglianese-Woody (Creator)
Appalachian State University (ASU )
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Abstract: PREMISE: Microsatellite markers were developed in the federally endangered species Liatris helleri (Asteraceae) to evaluate species boundaries with closely related congeners within the genus. METHODS AND RESULTS: Using Illumina data, 17 primer pairs were developed in populations of L. helleri. The primers amplified motifs from tri- to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross-amplification in L.aspera, L. microcephala, and L. pycnostachya. CONCLUSIONS: The developed primers for L. helleri serve as a novel genetic tool for future investigations in this genus, allowing for more explicit species delineation as well as population genetic analyses.

Additional Information

Clark, L. C., M. R. Gaglianese-Woody, and M. C. Estep. 2019. Development of 17 microsatellite markers in the federally endangered species Liatris helleri (Asteraceae). Applications in Plant Sciences 7(7): e1250. Publisher version of record available at:
Language: English
Date: 2019
Asteraceae, endangered species, Liatris helleri, perennial herb, Southern Appalachians, species boundaries

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