2,3,5-Triphenyltetrazolium chloride : effect on neurospora crassa

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Donna Galeazzi Wallace (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
James Wilson

Abstract: The tetrazollum salt, 2,3,5-triphenyltetrazollum chloride (TTC), inhibits germination of conidia of all Neurospora crassa strains tested in concentrations exceeding 600 ug/ml. At 50 ug/ml there is a dichotomous response to the salt. Some strains are completely inhibited; others, completely resistant. Tetrad analyses of crosses between resistant and sensitive strains provided evidence for the existence of both nuclear and extrachromosomal determinants of the dichotomous response. The nuclear determinants (TTCr , TTCs) are alleles of a single gene, closely linked to mating type In linkage group I. The extrachromosomal determinant is associated with the maternally inherited characteristics of (mi-l) , and, to a lesser extent, with those of [mi-2] and [ mi-4]. In the St. Lawrence-Oak Ridge strains and the Emerson strains mating type A Is TTCr, making type a is TTCs. Both mating types of the Rockefeller-Llndegren strains are TTCr. An [mi-1] strain with a TTCr nuclear allele is more resistant than an [mi-1] strain with a TTCS allele, suggesting a different mechanism for nuclear and cytoplasmic-based resistance.

Additional Information

Publication
Thesis
Language: English
Date: 1970
Subjects
Neurospora crassa $x Experiments
Genetics $x Experiments

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