The Antibacterial Efficacy Of Ethanolic Whole-Leaf Moringa Oleifera Sub-Fractions On Escherichia Coli

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Tanner Marc Orders (Creator)
Institution
Appalachian State University (ASU )
Web Site: https://library.appstate.edu/
Advisor
Chishimba Nathan Mowa

Abstract: In an effort to provide safer alternative options to pharmaceutical-based antibacterial treatment for infection-induced preterm labor, we have previously used whole-leaf extracts and high performance liquid chromatography (HPLC)-isolated subfractions of hydroethanolic Moringa oleifera (MO) to screen the antibacterial efficacy of MO against Escherichia coli (E. coli), a leading cause of urinary tract infections associated with preterm labor. These earlier studies found the ethanolic whole-leaf extract as well as three subfractions out of eight, to be the most potent. Here, we test the potency of each sub-fraction as well as investigate whether the antibacterial effects of the most potent leaf sub-fractions act synergistically, using in vitro liquid broth assays in a dose-dependent manner. Of the eight sub-fraction screened, sub-fraction five exerted the most antibacterial efficacy against E. coli. No significant synergistic antibacterial activity was observed between subfractions four, five, and six. We conclude that the anti-bacterial activity of whole leaf M. oleifera is exerted by the potent subfractions in a non-synergistic manner. Ongoing studies in our lab are testing the antibacterial potential of MO whole seed extracts.

Additional Information

Publication
Honors Project
Orders, T. (2018). "The Antibacterial Efficacy Of Ethanolic Whole-Leaf Moringa Oleifera Sub-Fractions On Escherichia Coli." Unpublished Honors Thesis. Appalachian State University, Boone, NC.
Language: English
Date: 2018
Keywords
Moringa Oleifera, Escherichia Coli, Antibacterial, Phytochemicals, Pre-Term Birth

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