Methanolinea Mesophila Sp. Nov., A Hydrogenotrophic Methanogen Isolated From Rice Field Soil, And Proposal Of The Archaeal Family Methanoregulaceae Fam. Nov. Within The Order Methanomicrobiales
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Suzanna Brauer Ph.D., Associate Professor (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
Abstract: A novel mesophilic, hydrogenotrophic methanogen, designated strain TNRT, was isolated from an anaerobic, propionate-degradation enrichment culture that was originally established from a ricefield soil sample from Taiwan. Cells were non-motile rods, 2.0–6.5 mm long by 0.3 mm wide.Filamentous (up to about 100 mm) and coccoid (about 1 mm in diameter) cells were also observed in cultures in the late exponential phase of growth. Strain TNRT grew at 20–40 6C (optimally at 37 6C), at pH 6.5–7.4 (optimally at pH 7.0) and in the presence of 0–25 g NaCl l”1 (optimally at 0 g NaCl l”1). The strain utilized H2/CO2 and formate for growth and produced methane. The G+C content of the genomic DNA was 56.4 mol%. Based on sequences of both the 16S rRNA gene andthe methanogen-specific marker gene mcrA, strain TNRT was related most closely to Methanolineatarda NOBI-1T; levels of sequence similarities were 94.8 and 86.4%, respectively. The 16S rRNAgene sequence similarity indicates that strain TNRT and M. tarda NOBI-1T represent differentspecies within the same genus. This is supported by shared phenotypic properties, includingsubstrate usage and cell morphology, and differences in growth temperature. Based on thesegenetic and phenotypic properties, strain TNRT is considered to represent a novel species of thegenus Methanolinea, for which the name Methanolinea mesophila sp. nov. is proposed; the type strain is TNRT (5NBRC 105659T5DSM 23604T). In addition, we also suggest family status for the E1/E2 group within the order Methanomicrobiales, for which the name Methanoregulaceae fam.nov. is proposed; the type genus of family is Methanoregula.
Methanolinea Mesophila Sp. Nov., A Hydrogenotrophic Methanogen Isolated From Rice Field Soil, And Proposal Of The Archaeal Family Methanoregulaceae Fam. Nov. Within The Order Methanomicrobiales
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Additional Information
- Publication
- Sakai S, Ehara M, Tseng I-C, Yamaguchi T, Bräuer Suzanna L, Cadillo-Quiroz H, Zinder SH and Imachi H. (2011).Methanolinea Mesophila Sp. Nov., A Hydrogenotrophic Methanogen Isolated From Rice Field Soil, And Proposal Of The Archaeal Family Methanoregulaceae Fam. Nov. Within The Order Methanomicrobiales. International Journal of Systematic and Evolutionary Microbiology, 62 (6):1389-95. (ISSN: 1466-5026) [DOI 10.1099/ijs.0.035048-0] Version Of Record Available At www.microbiologyresearch.org
- Language: English
- Date: 2012
- Keywords
- mesophilic, hydrogenotrophic methanogen, tnrt, anaerobic, propionate-degradation, Filamentous and coccoid cells