Spermidine Regulates Vibrio Cholerae Biofilm Formation Via Transport And Signaling Pathways

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Ece Karatan Ph.D., Associate Professor (Creator)
Appalachian State University (ASU )
Web Site: https://library.appstate.edu/

Abstract: Vibrio cholerae, the causative agent of the devastating diarrheal disease cholera, canform biofilms on diverse biotic and abiotic surfaces. Biofilm formation isimportant for the survival of this organism both in its natural environment andin the human host. Development of V. cholerae biofilms are regulated by complex regulatory networks that respond to environmental signals. One of these signals, norspermidine, is a polyamine that enhances biofilm formation via the NspS/MbaA signaling system. In this work, we have investigated the role of thepolyamine spermidine in regulating biofilm formation in V. cholerae. We showthat spermidine import requires PotD1, an ortholog of the periplasmic substratebinding protein of the spermidine transport system in Escherichia coli. We also show that deletion of the potD1 gene results in a significant increase in biofilm formation. We hypothesize that spermidine imported into the cell hinders biofilm formation. Exogenous spermidine further reduces biofilm formation in a PotD1-independent, but NspS/MbaA-dependent, manner. Our results suggest that polyamines affect biofilm formation in V. cholerae via multiple pathways involving both transport and signaling networks.

Additional Information

Marcus W. McGinnis, Zachary M. Parker, Nicholas E. Walter, Alex C. Rutkovsky, Claudia Cartaya-Marin, and Ece Karatan. (2009). "Spermidine Regulates Vibrio Cholerae Biofilm Formation Via Transport And Signaling Pathways." Microbiology Letters 299(2):166-74 (ISSN: 0378-1097) [DOI:10.1111/j.1574-6968.2009.01744.x ] Version Of Record Available At www.interscience.wiley.com
Language: English
Date: 2009
spermidine, vibrio cholerae, disease cholera, canform biofilms, diverse biotic and abiotic

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